Repeat the process of adjusting on the other channel.
The key is to avoid changes that alter the significant data in the image, rather than shifting the brightness levels.
These changes are equivalent to changes you could achieve by altering the camera settings when you took the photo. If you adjust, make a note of the settings you have selected (in this case Min=19, max=148). I have reduced the maximum slider to brighten the image (don’t take it too far or you will lose any graduation in brightness in the brighter areas) and I have moved the minimum slider a little to remove some of the background (again take care that you do not remove any meaningful information in this process. In this case the red channel seems a little dull. Have a play with the settings to get a feel for how they work. Select one of the images and use the Image::Adjust::Brightnes/Contrast menu to adjust the brightness, contrast etc. Note that you could use 3 files and merge red, blue and green channels just as easily.įor convenience I have maximised the ImageJ main window to fill the screen and used the Window::Tile menu to organise the two images. By default these will open as separate floating windows. Let us assume you have opened two files called red.jpg and blue.jpg. Use the menu File::Open (or use the shortcud Ctrl-O) and locate and open the first image file. In the folder where you unpacked Fiji you will find the ImageJ application. The following notes were done using the Windows version on a Mac there will be cosmetic changes in the appearance of the application and dialogues, but the processes should be essentially the same.
IMAGEJ MERGE CHANNELS PORTABLE
ImageJ and Fiji come as a portable application – ie you do not need to install, just unpack the download and run the program from wherever you downloaded it. If you don’t have the program, you can get it from.
IMAGEJ MERGE CHANNELS FREE
Image optimisation and merging can be achieved easily using the free Fiji package with ImageJ.
Commonly you will want to merge these images into a composite. If you are using fluorescence microscopy you may need to merge images taken with different filter sets – for example DAPI to pick out nuclei together with fluorescent staining with or or more specific antibodies.